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How to the Research Time of 8-hydroxyquinoline Copper?

How to the Research Time of 8-hydroxyquinoline Copper?

Cells from a growing culture were harvested and treated in the presence or absence of 8-hydroxyquinoline Copper WSDTY) with 0.6 μM 8HQ or NQ in HdBSM at 37°C. The OD600 of the samples was 0.04. Aliquots were removed at the indicated treatment times. The samples were then diluted 10-fold in 7H9SM. Aliquots (5 μl) of each dilution were spotted on 7H10 agar plates and incubated at 37°C for 11 days before pictures were taken.

Dose matrix experiments.Serial dilutions (2-fold) of CuCl2 or ZnCl2 were carried out at 4-fold concentrations in 96-well plate rows, and one-fourth of the total volume of each well was filled with these dilutions. Test compound was diluted separately in a 12-well reservoir at 4-fold concentrations, and then one-fourth volume of each well was added into the wells of the respective columns containing the metal solutions or water, giving rise to a dose matrix. Finally, one-half volume of 2-fold-concentrated HdBSM with cells (OD600 = 0.04) was added to each well. The final concentrations of Cu and compounds are given in the appropriate figures. Plates were incubated at 37°C for 7 days, after which the resazurin assay was used to determine the growth of the cells by measuring the fluorescence intensity using a Cytation3 imaging reader.

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